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Registros recuperados: 11
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Antibiotic resistance and integrons in Shiga toxin-producing Escherichia coli (STEC) BJM
Colello,Rocío; Etcheverría,Analía I.; Conza,Jose A. Di; Gutkind,Gabriel O.; Padola,Nora L..
Shiga toxin-producing Escherichia coli (STEC) cause hemorrhagic colitis (HC) and hemolytic-uremic syndrome in humans (HUS). Cattle are the main reservoir of STEC and transmission to humans occurs through contaminated food and water. Antibiotics are used in pig production systems to combat disease and improve productivity and play a key role in the dissemination of antibiotic resistance genes to the bacteria. Integrons have been identified in resistant bacteria allowing for the acquisition and dissemination of antibiotic resistance genes. STEC strains isolated from humans and animals have developed antibiotic resistance. In our laboratory, 21 non-157 STEC strains isolated from pigs were analyzed to detect class 1 and 2 integrons by PCR. Eight carried...
Tipo: Info:eu-repo/semantics/article Palavras-chave: STEC; Integrons; Multiresistance; Antibiotics; Public health.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000100001
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Anti-tuberculosis drug resistance in strains of Mycobacterium tuberculosis isolated from patients in a tertiary hospital in Bahia BJID
Matos,Eliana Dias; Lemos,Antônio Carlos Moreira; Bittencourt,Carolina; Mesquita,Cristiane Leite.
Anti-tuberculosis drug resistance, particularly multiresistance, is a crucial issue in the control of tuberculosis (TB). This study estimated the prevalence of primary and acquired anti-tuberculosis drug resistance in strains of Mycobacterium tuberculosis isolated from hospitalized patients, to identify the risk factors for resistance, and to evaluate the its impact on hospital mortality for tuberculosis. Strains of Mycobacterium tuberculosis from 217 patients hospitalized for TB were analyzed. Subjects were recruited sequentially at a TB reference hospital in Salvador, Bahia, Brazil from July 2001 to July 2003. Multiresistant (MR) strains were defined as strains resistant to rifampicin and isoniazid. Of a total of 217 strains isolated, 41 (19.0%, 95%CI:...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Mycobacterium tuberculosis; Tuberculosis; Resistance; Multiresistance; Hospitals; Bahia-Brazil.
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702007000300007
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Biofilm production by clinical isolates of Pseudomonas aeruginosa and structural changes in LasR protein of isolates non biofilm-producing BJID
Lima,Jailton Lobo da Costa; Alves,Lilian Rodrigues; Jacomé,Paula Regina Luna de Araújo; Bezerra Neto,João Pacífico; Maciel,Maria Amélia Vieira; Morais,Marcia Maria Camargo de.
ABSTRACT Introduction: Biofilm production is an important mechanism for the survival of Pseudomonas aeruginosa and its relationship with antimicrobial resistance represents a challenge for patient therapeutics. P. aeruginosa is an opportunistic pathogen frequently associated to nosocomial infections, especially in imunocompromised hosts. Objectives: Analyze the phenotypic biofilm production in P. aeruginosa isolates, describe clonal profiles, and analyze quorum sensing (QS) genes and the occurrence of mutations in the LasR protein of non-biofilm producing isolates. Methods: Isolates were tested for biofilm production by measuring cells adherence to the microtiter plates. Clonal profile analysis was carried out through ERIC-PCR, QS genes were by specific...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Pseudomonas aeruginosa; Biofilm; Multiresistance; Quorum sensing (QS).
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702018000200129
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Genetic similarity between staphylococcus sp isolated from human and hospital settings, and susceptibility to different antimicrobials BJM
Fabiano,Telma Luciana Trovó; Ávila,Bruna Helena Pinheiro de; Dias,Cleusa Cascaes; Maluta,Renato Pariz; Ávila,Fernando Antonio de.
One hundred and forty-three samples from human hands and hospital beds were collected at a teaching hospital in the city of Ribeirão Preto/SP by swabs, and placed in BHI broth. Following a 24 h incubation period at 37ºC, they were seeded on Petri dishes containing Agar "Staphylococcus Medium 110". Colonies typical of the genus Staphylococcus were collected and stored at 4ºC until tested for catalase, mannitol, hemolysis, DNAse and coagulase. Strains were analyzed by RAPD-PCR to verify their similarity, and tested for sensitivity to ten different antibiotics. From the ninety-two isolated strains, 67 (72,8%) were coagulase- negative and 25 (27,2%) coagulase-positive. Similarity analysis showed a great heterogeneity among strains, but some presented 100%...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Similarity; RAPD-PCR; Multiresistance.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822008000400010
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Metallo-β-lactamase-producing Pseudomonas aeruginosa in two hospitals from southern Brazil BJID
Wirth,Fernanda W.; Picoli,Simone U.; Cantarelli,Vlademir V.; Gonçalves,Ana L.S.; Brust,Flávia R.; Santos,Liege M.O.; Barreto,Michelle F..
This study determined the prevalence of metallo-β-lactamase (MBL)-producing Pseudomonas aeruginosa in two hospitals located in the Southern part of Brazil and compare the performance of two different phenotypic tests. Thirty-one non-repetitive Pseudomonas aeruginosa isolates from various clinical samples from patients admitted to two hospitals located in Rio Grande do Sul, Brazil (twenty-three from a hospital in Porto Alegre City and eight isolates from a hospital in Vale dos Sinos Region). All strains suggestive of possessing MBLs by phenotypic methods were included in this study. Phenotypic detection of MBLs was carried out simultaneously by using both the MBL Etest® and disk approximation test using 2-mercaptopropionic acid close to a ceftazidime disk....
Tipo: Info:eu-repo/semantics/article Palavras-chave: Metallo-beta-lactamase; Pseudomonas aeruginosa; Multiresistance.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702009000300003
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Multidrug efflux systems in Escherichia coli and Enterobacter cloacae obtained from wholesome broiler carcasses BJM
Moreira,Maria Aparecida S.; Rodrigues,Patrícia P.C.F.; Tomaz,Rafael S.; Moraes,Célia A. de.
Members of the Enterobacteriaceae family are present in the intestines of man and animals as commensals or are important disease causing agents. Bacteria bearing multidrug efflux systems (MDR) are able to survive adverse ecological niches. Multiresistant Escherichia coli and Enterobacter cloacae isolates from wholesome broiler carcasses were investigated for the presence of MDR. Lowering of Minimal Inhibitory Concentration for antimicrobials in the presence of a proton-motive force (PMF) uncoupler was tested as a potential display of the MDR phenotype. PCR amplification of the genes encoding AcrA and AcrB, components of a MDR system was performed. Diversity of each species was ascertained by Pulsed-Field Gel Electrophoresis (PFGE) of DNA digested with...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Multiresistance; Antimicrobials; Enterobacteriaceae; Proton-motive force; Diversity.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822009000200007
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Multidrug efflux systems in Gram-negative bacteria BJM
Moreira,Maria Aparecida Scatamburlo; Souza,Edmar Chartone de; Moraes,Célia Alencar de.
Multidrug efflux mechanisms in bacteria contribute significantly to intrinsic and acquired resistance to antimicrobial agents. Genome analysis have confirmed the broad distribution of these systems in Gram-negative as well as in Gram-positive bacteria. Among resistance mechanisms, the multidrug efflux system or pump deserves special attention, since a cell that has acquired it can simultaneously diminish or even suppress the susceptibility to a wide range of antimicrobials. The efflux system is mediated by transport proteins which confer resistance to toxic compounds. In Gram-negative bacteria, a tripartite efflux system is necessary to expel the drug to the outer medium: a protein localized in the cytoplasmic membrane; another in the periplasmatic space...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Gram-negative bacteria; Multidrug efflux systems; Antimicrobials; Transport proteins; Multiresistance.
Ano: 2004 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822004000100003
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Nondiphtherial Corynebacterium species isolated from clinical specimens of patients in a university hospital, Rio de Janeiro, Brazil BJM
Camello,Thereza Cristina Ferreira; Mattos-Guaraldi,Ana Luiza; Formiga,Luiz Carlos Duarte; Marques,Elizabeth Andrade.
Over a five-year period, 163 strains of Corynebacterium sp. were recovered from different clinical specimens of patients from a Brazilian University hospital. Genitourinary tract and intravenous sites specimens were the most frequent sources of corynebacteria (46.62%). Corynebacterium amycolatum (29.55%), Corynebacterium minutissimum (20.45%) and Corynebacterium pseudodiphtheriticum (13.63%) were the predominant species found in genitourinary tract. C. minutissimum (24.14%) and Corynebacterium propinquum (17.24%) in surgical and/or other skin wounds and abscesses; Corynebacterium xerosis (25%), C. amycolatum (21.87%) and C. pseudodiphtheriticum (18.75%) in intravenous sites; C. pseudodiphtheriticum (33.33%) and C. propinquum (33.33%) in lower respiratory...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Corynebacterium sp; Nondiphtherial corynebacteria; Nosocomial infections; Multiresistance.
Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822003000100009
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Occurrence and antimicrobial resistance of bacteria in retail market spices Ciência Rural
Costa,Milena da Cruz; Cruz,Alexsandra Iarlen Cabral; Bispo,Aline Simões da Rocha; Ferreira,Mariza Alves; Costa,João Albany; Evangelista-Barreto,Norma Suely.
ABSTRACT: This study aimed to evaluate the microbiological quality and the transmission of multidrug-resistant bacteria in different spices sold in town fairs (local food markets) in the municipalities of Recôncavo Baiano. Samples of black pepper, oregano, and cinnamon were collected over a period of six months and investigated for coliforms at 45 °C, Staphylococcus spp., Staphylococcus aureus, Bacillus spp., Bacillus cereus, Escherichia coli and Salmonella spp. The contamination in the black pepper samples (log 4.66 CFU g-1) was higher (P>0.05), than those of cinnamon (log 2.55 CFU g-1) and oregano (log 2.49 CFU g-1), particularly for B. cereus. E. coli (89%) and Salmonella spp. (67%) were isolated only from black pepper. B. cereus and S. aureus showed...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Spice plants; Antibacterial activity; Multiresistance.
Ano: 2020 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782020000400455
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The presence of plasmids in Aeromonas hydrophila and its relationship with antimicrobial and heavy metal-resistance profiles Ciência Rural
Freitas,Mariana Ramos; Freire,Naiana Braga; Peixoto,Luciana Jatobá e Silva; Oliveira,Samira Teixeira Leal de; Souza,Renilde Cordeiro de; Gouveia,João José de Simoni; Costa,Mateus Matiuzzi da; Gouveia,Gisele Veneroni.
ABSTRACT: Aeromonas hydrophila is a common fish pathogen that causes extensive damage to aquaculture. To develop and implement a more adequate strategy to farm fish, it is crucial to understand the bacterial-resistance levels and their transference dynamics. The objective of this study was to analyze the resistance profile of isolated Aeromonas hydrophila to antimicrobial agents and heavy metals and draw a correlation of the observed profiles with the presence of plasmids. Resistance of the isolated bacteria to antimicrobial agents (oxacilin, gentamicin, tetracycline, and nalidixic acid) and heavy metals (cadmium, lead, copper, and manganese) was verified using the minimum bactericidal concentration (MBC) and minimum inhibitory concentration (MIC)...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Aquiculture; Multiresistance; Megaplasmids; Cromossomic DNA; Integrons.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782018000900451
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Trends in bacterial resistance in a tertiary university hospital over one decade BJID
Góngora Rubio,Fernando; Oliveira,Viviane Decicera Colombo; Rangel,Regina Mara Custódio; Nogueira,Mara Corrêa Lelles; Almeida,Margarete Teresa Gottardo.
The objective of this study was to investigate bacterial resistance trends, infection sites and the relationship between resistance and admittance to the intensive care unit (ICU). A total of 53,316 bacteria identified between 1999 and 2008 were evaluated. Multidrug resistance was characterized when gram-negative bacilli (GNB) presented resistance to two or more classes of antibiotics. Gram-positive cocci (CPC) were assessed for resistance to penicillin, oxacillin and vancomycin. GNB were the most common (66.1%) isolate. There was a 3.7-fold overall increase in multidrug resistant GNB over the study period; Acinetobacter baumanii and Staphylococcus aureus were the most prevalent. Highest increases were recorded for Kleb siella pneumoniae (14.6-fold) and...
Tipo: Info:eu-repo/semantics/other Palavras-chave: Multiresistance; Bacteria; Trends.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702013000400014
Registros recuperados: 11
Primeira ... 1 ... Última
 

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